Publications AG Klein

Werner-Klein, M., Grujovic, A., Irlbeck, C., Obradovic, M., Hoffmann, M., Koerkel-Qu, H., Lu, X., Treitschke, S., Kostler, C., Botteron, C., Weidele, K., Werno, C., Polzer, B., Kirsch, S., Guzvic, M., Warfsmann, J., Honarnejad, K., Czyz, Z., Feliciello, G., Blochberger, I., Grunewald, S., Schneider, E., Haunschild, G., Patwary, N., Guetter, S., Huber, S., Rack, B., Harbeck, N., Buchholz, S., Rummele, P., Heine, N., Rose-John, S., and Klein, C. A. (2020) Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during clinical latency. Nat Commun11, 4977, doi:10.1038/s41467-020-18701-4

Vollmann-Zwerenz, A., Leidgens, V., Feliciello, G., Klein, C. A., and Hau, P. (2020) Tumor Cell Invasion in Glioblastoma. Int J Mol Sci21, doi:10.3390/ijms21061932

Metzenmacher, M., Varaljai, R., Hegedus, B., Cima, I., Forster, J., Schramm, A., Scheffler, B., Horn, P. A., Klein, C. A., Szarvas, T., Reis, H., Bielefeld, N., Roesch, A., Aigner, C., Kunzmann, V., Wiesweg, M., Siveke, J. T., Schuler, M., and Lueong, S. S. (2020) Plasma Next Generation Sequencing and Droplet Digital-qPCR-Based Quantification of Circulating Cell-Free RNA for Noninvasive Early Detection of Cancer. Cancers (Basel)12, doi:10.3390/cancers12020353

Blazquez, R., Rietkotter, E., Wenske, B., Wlochowitz, D., Sparrer, D., Vollmer, E., Muller, G., Seegerer, J., Sun, X., Dettmer, K., Barrantes-Freer, A., Stange, L., Utpatel, K., Bleckmann, A., Treiber, H., Bohnenberger, H., Lenz, C., Schulz, M., Reimelt, C., Hackl, C., Grade, M., Buyuktas, D., Siam, L., Balkenhol, M., Stadelmann, C., Kube, D., Krahn, M. P., Proescholdt, M. A., Riemenschneider, M. J., Evert, M., Oefner, P. J., Klein, C. A., Hanisch, U. K., Binder, C., and Pukrop, T. (2020) LEF1 supports metastatic brain colonization by regulating glutathione metabolism and increasing ROS resistance in breast cancer. International journal of cancer146, 3170-3183, doi:10.1002/ijc.32742

Klein, C.A. Cancer progression and the invisible phase of metastatic colonization. Nat Rev Cancer 20, 681–694 (2020). https://doi.org/10.1038/s41568-020-00300-6

Werner-Klein, M., Grujovic, A., Irlbeck, C., Obradovic, M., Hoffmann, M., Koerkel-Qu, H., Lu, X., Treitschke, S., Kostler, C., Botteron, C., Weidele, K., Werno, C., Polzer, B., Kirsch, S., Guzvic, M., Warfsmann, J., Honarnejad, K., Czyz, Z., Feliciello, G., Blochberger, I., Grunewald, S., Schneider, E., Haunschild, G., Patwary, N., Guetter, S., Huber, S., Rack, B., Harbeck, N., Buchholz, S., Rummele, P., Heine, N., Rose-John, S., and Klein, C. A. (2020) Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during clinical latency. Nat Commun11, 4977, doi:10.1038/s41467-020-18701-4

Vollmann-Zwerenz, A., Leidgens, V., Feliciello, G., Klein, C. A., and Hau, P. (2020) Tumor Cell Invasion in Glioblastoma. Int J Mol Sci21, doi:10.3390/ijms21061932

Metzenmacher, M., Varaljai, R., Hegedus, B., Cima, I., Forster, J., Schramm, A., Scheffler, B., Horn, P. A., Klein, C. A., Szarvas, T., Reis, H., Bielefeld, N., Roesch, A., Aigner, C., Kunzmann, V., Wiesweg, M., Siveke, J. T., Schuler, M., and Lueong, S. S. (2020) Plasma Next Generation Sequencing and Droplet Digital-qPCR-Based Quantification of Circulating Cell-Free RNA for Noninvasive Early Detection of Cancer. Cancers (Basel)12, doi:10.3390/cancers12020353

Blazquez, R., Rietkotter, E., Wenske, B., Wlochowitz, D., Sparrer, D., Vollmer, E., Muller, G., Seegerer, J., Sun, X., Dettmer, K., Barrantes-Freer, A., Stange, L., Utpatel, K., Bleckmann, A., Treiber, H., Bohnenberger, H., Lenz, C., Schulz, M., Reimelt, C., Hackl, C., Grade, M., Buyuktas, D., Siam, L., Balkenhol, M., Stadelmann, C., Kube, D., Krahn, M. P., Proescholdt, M. A., Riemenschneider, M. J., Evert, M., Oefner, P. J., Klein, C. A., Hanisch, U. K., Binder, C., and Pukrop, T. (2020) LEF1 supports metastatic brain colonization by regulating glutathione metabolism and increasing ROS resistance in breast cancer. International journal of cancer146, 3170-3183, doi:10.1002/ijc.32742

Werner-Klein, M., Scheitler, S., Hoffmann, M., Hodak, I., Dietz, K., Lehnert, P., Naimer, V., Polzer, B., Treitschke, S., Werno, C., Markiewicz, A., Weidele, K., Czyz, Z., Hohenleutner, U., Hafner, C., Haferkamp, S., Berneburg, M., Rummele, P., Ulmer, A., and Klein, C. A. (2018) Genetic alterations driving metastatic colony formation are acquired outside of the primary tumour in melanoma. Nat Commun9, 595, doi:10.1038/s41467-017-02674-y

Ulmer, A., Dietz, K., Werner-Klein, M., Hafner, H. M., Schulz, C., Renner, P., Weber, F., Breuninger, H., Rocken, M., Garbe, C., Fierlbeck, G., and Klein, C. A. (2018) The sentinel lymph node spread determines quantitatively melanoma seeding to non-sentinel lymph nodes and survival. European journal of cancer91, 1-10, doi:10.1016/j.ejca.2017.12.002

Luke, F., Blazquez, R., Yamaci, R. F., Lu, X., Pregler, B., Hannus, S., Menhart, K., Hellwig, D., Wester, H. J., Kropf, S., Heudobler, D., Grosse, J., Moosbauer, J., Hutterer, M., Hau, P., Riemenschneider, M. J., Bayerlova, M., Bleckmann, A., Polzer, B., Beissbarth, T., Klein, C. A., and Pukrop, T. (2018) Isolated metastasis of an EGFR-L858R-mutated NSCLC of the meninges: the potential impact of CXCL12/CXCR4 axis in EGFRmut NSCLC in diagnosis, follow-up and treatment. Oncotarget9, 18844-18857, doi:10.18632/oncotarget.24787

Lorentzen, A., Becker, P. F., Kosla, J., Saini, M., Weidele, K., Ronchi, P., Klein, C., Wolf, M. J., Geist, F., Seubert, B., Ringelhan, M., Mihic-Probst, D., Esser, K., Roblek, M., Kuehne, F., Bianco, G., O'Connor, T., Muller, Q., Schuck, K., Lange, S., Hartmann, D., Spaich, S., Gross, O., Utikal, J., Haferkamp, S., Sprick, M. R., Damle-Vartak, A., Hapfelmeier, A., Huser, N., Protzer, U., Trumpp, A., Saur, D., Vartak, N., Klein, C. A., Polzer, B., Borsig, L., and Heikenwalder, M. (2018) Single cell polarity in liquid phase facilitates tumour metastasis. Nat Commun9, 887, doi:10.1038/s41467-018-03139-6

Hoffmann, M., Pasch, S., Schamberger, T., Maneck, M., Mohlendick, B., Schumacher, S., Brockhoff, G., Knoefel, W. T., Izbicki, J., Polzer, B., Stoecklein, N. H., and Klein, C. A. (2018) Diagnostic pathology of early systemic cancer: ERBB2 gene amplification in single disseminated cancer cells determines patient survival in operable esophageal cancer. International journal of cancer142, 833-843, doi:10.1002/ijc.31108

Harper, K. L., Sosa, M. S., Entenberg, D., Hosseini, H., Cheung, J. F., Nobre, R., Avivar-Valderas, A., Nagi, C., Girnius, N., Davis, R. J., Farias, E. F., Condeelis, J., Klein, C. A., and Aguirre-Ghiso, J. A. (2018) Corrigendum: Mechanism of early dissemination and metastasis in Her2(+) mammary cancer. Nature553, 366, doi:10.1038/nature24666

Schumacher, S., Bartenhagen, C., Hoffmann, M., Will, D., Fischer, J. C., Baldus, S. E., Vay, C., Fluegen, G., Dizdar, L., Vallbohmer, D., Klein, C. A., Knoefel, W. T., Stoecklein, N. H., and Mohlendick, B. (2017) Disseminated tumour cells with highly aberrant genomes are linked to poor prognosis in operable oesophageal adenocarcinoma. British journal of cancer117, 725-733, doi:10.1038/bjc.2017.233

Suzuki, T., Asami, M., Hoffmann, M., Lu, X., Guzvic, M., Klein, C. A., and Perry, A. C. (2016) Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes. Nat Commun7, 12676, doi:10.1038/ncomms12676

Hosseini, H., Obradovic, M. M., Hoffmann, M., Harper, K. L., Sosa, M. S., Werner-Klein, M., Nanduri, L. K., Werno, C., Ehrl, C., Maneck, M., Patwary, N., Haunschild, G., Guzvic, M., Reimelt, C., Grauvogl, M., Eichner, N., Weber, F., Hartkopf, A. D., Taran, F. A., Brucker, S. Y., Fehm, T., Rack, B., Buchholz, S., Spang, R., Meister, G., Aguirre-Ghiso, J. A., and Klein, C. A. (2016) Early dissemination seeds metastasis in breast cancer. Nature540, 552-558, doi:10.1038/nature20785

Harper, K. L., Sosa, M. S., Entenberg, D., Hosseini, H., Cheung, J. F., Nobre, R., Avivar-Valderas, A., Nagi, C., Girnius, N., Davis, R. J., Farias, E. F., Condeelis, J., Klein, C. A., and Aguirre-Ghiso, J. A. (2016) Mechanism of early dissemination and metastasis in Her2+ mammary cancer. Nature540, doi:10.1038/nature20609

Alberter, B., Klein, C. A., and Polzer, B. (2016) Single-cell analysis of CTCs with diagnostic precision: opportunities and challenges for personalized medicine. Expert Rev Mol Diagn16, 25-38, doi:10.1586/14737159.2016.1121099

Frances, D., Sharma, N., Pofahl, R., Maneck, M., Behrendt, K., Reuter, K., Krieg, T., Klein, C. A., Haase, I., and Niemann, C. (2015) A role for Rac1 activity in malignant progression of sebaceous skin tumors. Oncogene34, 5505-5512, doi:10.1038/onc.2014.471

Czyz, Z. T., and Klein, C. A. (2015) Deterministic Whole-Genome Amplification of Single Cells. Methods Mol Biol1347, 69-86, doi:10.1007/978-1-4939-2990-0_5

Werner-Klein, M., Proske, J., Werno, C., Schneider, K., Hofmann, H. S., Rack, B., Buchholz, S., Ganzer, R., Blana, A., Seelbach-Gobel, B., Nitsche, U., Mannel, D. N., and Klein, C. A. (2014) Immune humanization of immunodeficient mice using diagnostic bone marrow aspirates from carcinoma patients. PloS one9, e97860, doi:10.1371/journal.pone.0097860

Wang, J., Lu, X., Sakk, V., Klein, C. A., and Rudolph, K. L. (2014) Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres. Blood124, 3237-3240, doi:10.1182/blood-2014-04-568055

Vay, C., Hosch, S. B., Stoecklein, N. H., Klein, C. A., Vallbohmer, D., Link, B. C., Yekebas, E. F., Izbicki, J. R., Knoefel, W. T., and Scheunemann, P. (2014) Integrin expression in esophageal squamous cell carcinoma: loss of the physiological integrin expression pattern correlates with disease progression. PloS one9, e109026, doi:10.1371/journal.pone.0109026

Ulmer, A., Dietz, K., Hodak, I., Polzer, B., Scheitler, S., Yildiz, M., Czyz, Z., Lehnert, P., Fehm, T., Hafner, C., Schanz, S., Rocken, M., Garbe, C., Breuninger, H., Fierlbeck, G., and Klein, C. A. (2014) Quantitative measurement of melanoma spread in sentinel lymph nodes and survival. PLoS medicine11, e1001604, doi:10.1371/journal.pmed.1001604

Renner, P., Rovira, J., Klein, C., Schlitt, H. J., Geissler, E. K., and Kroemer, A. (2014) KLRG1(+) natural killer cells protect against pulmonary metastatic disease by immunosurveillance. Oncoimmunology3, e28328, doi:10.4161/onci.28328

Polzer, B., Medoro, G., Pasch, S., Fontana, F., Zorzino, L., Pestka, A., Andergassen, U., Meier-Stiegen, F., Czyz, Z. T., Alberter, B., Treitschke, S., Schamberger, T., Sergio, M., Bregola, G., Doffini, A., Gianni, S., Calanca, A., Signorini, G., Bolognesi, C., Hartmann, A., Fasching, P. A., Sandri, M. T., Rack, B., Fehm, T., Giorgini, G., Manaresi, N., and Klein, C. A. (2014) Molecular profiling of single circulating tumor cells with diagnostic intention. EMBO molecular medicine6, 1371-1386, doi:10.15252/emmm.201404033

Neves, R. P., Raba, K., Schmidt, O., Honisch, E., Meier-Stiegen, F., Behrens, B., Mohlendick, B., Fehm, T., Neubauer, H., Klein, C. A., Polzer, B., Sproll, C., Fischer, J. C., Niederacher, D., and Stoecklein, N. H. (2014) Genomic high-resolution profiling of single CKpos/CD45neg flow-sorting purified circulating tumor cells from patients with metastatic breast cancer. Clin Chem60, 1290-1297, doi:10.1373/clinchem.2014.222331

Guzvic, M., Braun, B., Ganzer, R., Burger, M., Nerlich, M., Winkler, S., Werner-Klein, M., Czyz, Z. T., Polzer, B., and Klein, C. A. (2014) Combined genome and transcriptome analysis of single disseminated cancer cells from bone marrow of prostate cancer patients reveals unexpected transcriptomes. Cancer research74, 7383-7394, doi:10.1158/0008-5472.CAN-14-0934

Czyz, Z. T., Hoffmann, M., Schlimok, G., Polzer, B., and Klein, C. A. (2014) Reliable Single Cell Array CGH for Clinical Samples. PloS one9, e85907, doi:10.1371/journal.pone.0085907

Polzer, B., and Klein, C. A. (2013) Metastasis awakening: the challenges of targeting minimal residual cancer. Nature medicine19, 274-275, doi:10.1038/nm.3121

Klein, C. A. (2013) Selection and adaptation during metastatic cancer progression. Nature501, 365-372, doi:10.1038/nature12628

Hittinger, M., Czyz, Z. T., Huesemann, Y., Maneck, M., Botteron, C., Kaeufl, S., Klein, C. A., and Polzer, B. (2013) Molecular profiling of single Sca-1+/CD34+,- cells--the putative murine lung stem cells. PloS one8, e83917, doi:10.1371/journal.pone.0083917

Guzvic, M., and Klein, C. A. (2013) Cancer dormancy: time to explore its clinical relevance. Breast Cancer Res15, 321, doi:10.1186/bcr3590

Fischer, J. C., Niederacher, D., Topp, S. A., Honisch, E., Schumacher, S., Schmitz, N., Zacarias Fohrding, L., Vay, C., Hoffmann, I., Kasprowicz, N. S., Hepp, P. G., Mohrmann, S., Nitz, U., Stresemann, A., Krahn, T., Henze, T., Griebsch, E., Raba, K., Rox, J. M., Wenzel, F., Sproll, C., Janni, W., Fehm, T., Klein, C. A., Knoefel, W. T., and Stoecklein, N. H. (2013) Diagnostic leukapheresis enables reliable detection of circulating tumor cells of nonmetastatic cancer patients. Proceedings of the National Academy of Sciences of the United States of America110, 16580-16585, doi:10.1073/pnas.1313594110

Luebke, A. M., Baudis, M., Matthaei, H., Vashist, Y. K., Verde, P. E., Hosch, S. B., Erbersdobler, A., Klein, C. A., Izbicki, J. R., Knoefel, W. T., and Stoecklein, N. H. (2012) Losses at chromosome 4q are associated with poor survival in operable ductal pancreatic adenocarcinoma. Pancreatology12, 16-22, doi:10.1016/j.pan.2011.11.001

Lichtenauer, U., Shapiro, I., Sackmann, S., Drouin, J., Scheele, J., Maneck, M., Klein, C., and Beuschlein, F. (2012) The side population phenomenon enriches for designated adrenocortical progenitor cells in mice. J Endocrinol215, 383-391, doi:10.1530/JOE-12-0393

Kirsch, S., and Klein, C. A. (2012) Sequence error storms and the landscape of mutations in cancer. Proceedings of the National Academy of Sciences of the United States of America109, 14289-14290, doi:10.1073/pnas.1212246109

Cucuruz, B., Dango, S., Jurinovic, V., Mayer, O., Follo, M., Bohm, J., Freudenberg, N., Elze, M., Sienel, W., Klein, C. A., Passlick, B., and Polzer, B. (2012) MAGE qPCR improves the sensitivity and accuracy of EBUS-TBNA for the detection of lymphatic cancer spread. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer7, 690-697, doi:10.1097/JTO.0b013e31824294de

VerMilyea, M. D., Maneck, M., Yoshida, N., Blochberger, I., Suzuki, E., Suzuki, T., Spang, R., Klein, C. A., and Perry, A. C. (2011) Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres. Embo J30, 1841-1851, doi: 10.1038/emboj.2011.92

Klein, C. A. (2011) Framework models of tumor dormancy from patient-derived observations. Curr Opin Genet Dev21, 42-49, doi: 10.1016/j.gde.2010.10.011

Conrad, C., Husemann, Y., Niess, H., von Luettichau, I., Huss, R., Bauer, C., Jauch, K. W., Klein, C. A., Bruns, C., and Nelson, P. J. (2011) Linking transgene expression of engineered mesenchymal stem cells and angiopoietin-1-induced differentiation to target cancer angiogenesis. Ann Surg253, 566-571, doi: 10.1097/SLA.0b013e3181fcb5d8

Chambers, K. F., Pearson, J. F., Pellacani, D., Aziz, N., Guzvic, M., Klein, C. A., and Lang, S. H. (2011) Stromal upregulation of lateral epithelial adhesions: gene expression analysis of signalling pathways in prostate epithelium. J Biomed Sci18, 45, doi:10.1186/1423-0127-18-45

Stoecklein, N. H., and Klein, C. A. (2010) Genetic disparity between primary tumours, disseminated tumour cells, and manifest metastasis. International journal of cancer126, 589-598, doi: 10.1002/ijc.24916

Eberle, J., Spangler, B., Becker, J. C., Heinemann, S. H., Klein, C. A., Kunz, M., Kuphal, S., Langer, P., Mauch, C., Meierjohann, S., Paschen, A., Schadendorf, D., Schartl, M., Schittek, B., Schonherr, R., Tuting, T., Zigrino, P., and Bosserhoff, A. K. (2010) Multicentre study on standardisation of melanoma cell culture--an initiative of the German Melanoma Research Network. Pigment Cell Melanoma Res23, 296-298, doi: 10.1111/j.1755-148X.2010.00684.x

Dango, S., Wang, X. T., Gold, M., Cucuruz, B., Klein, C. A., Passlick, B., and Sienel, W. (2010) Expression of melanoma-antigen-A (MAGE-A) in disseminated tumor cells in regional lymph nodes of patients with operable non-small cell lung cancer. Lung Cancer67, 290-295, doi: 10.1016/j.lungcan.2009.04.012

Weckermann, D., Polzer, B., Ragg, T., Blana, A., Schlimok, G., Arnholdt, H., Bertz, S., Harzmann, R., and Klein, C. A. (2009) Perioperative activation of disseminated tumor cells in bone marrow of patients with prostate cancer. Journal of clinical oncology : official journal of the American Society of Clinical Oncology27, 1549-1556, doi: 10.1200/JCO.2008.17.0563

Klein, C. A., and Stoecklein, N. H. (2009) Lessons from an aggressive cancer: evolutionary dynamics in esophageal carcinoma. Cancer research69, 5285-5288, doi: 10.1158/0008-5472.can-08-4586

Klein, C. A., (2009) Parallel progression of primary tumours and metastases. Nat Rev Cancer9, 302-312, doi: 10.1038/nrc2627

Imle, A., Polzer, B., Alexander, S., Klein, C. A., and Friedl, P. (2009) Genomic instability of micronucleated cells revealed by single-cell comparative genomic hybridization. Cytometry. Part A : the journal of the International Society for Analytical Cytology75, 562-568, doi: 10.1002/cyto.a.20733

Husemann, Y., and Klein, C. A. (2009) The analysis of metastasis in transgenic mouse models. Transgenic Res18, 1-5, doi: 10.1007/s11248-008-9225-0

Hafner, C., Stoehr, R., van Oers, J. M., Zwarthoff, E. C., Hofstaedter, F., Klein, C., Landthaler, M., Hartmann, A., and Vogt, T. (2009) The absence of BRAF, FGFR3, and PIK3CA mutations differentiates lentigo simplex from melanocytic nevus and solar lentigo. The Journal of investigative dermatology129, 2730-2735, doi: 10.1038/jid.2009.146

Gires, O., Klein, C. A., and Baeuerle, P. A. (2009) On the abundance of EpCAM on cancer stem cells. Nat Rev Cancer9, 143; author reply 143, doi: 10.1038/nrc2499-c1

Stoecklein, N. H., Hosch, S. B., Bezler, M., Stern, F., Hartmann, C. H., Vay, C., Siegmund, A., Scheunemann, P., Schurr, P., Knoefel, W. T., Verde, P. E., Reichelt, U., Erbersdobler, A., Grau, R., Ullrich, A., Izbicki, J. R., and Klein, C. A. (2008) Direct genetic analysis of single disseminated cancer cells for prediction of outcome and therapy selection in esophageal cancer. Cancer cell13, 441-453, doi: 10.1016/j.ccr.2008.04.005

Sienel, W., Polzer, B., Elshawi, K., Lindner, M., Morresi-Hauf, A., Vay, C., Eder, F., Passlick, B., and Klein, C. A. (2008) Cellular localization of EMMPRIN predicts prognosis of patients with operable lung adenocarcinoma independent from MMP-2 and MMP-9. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc21, 1130-1138, doi: 10.1038/modpathol.2008.102

Klein, C. A. (2008) The direct molecular analysis of metastatic precursor cells in breast cancer: A chance for a better understanding of metastasis and for personalised medicine. European journal of cancer, doi: 10.1016/j.ejca.2008.09.035

Klein, C. A. (2008) Cancer. The metastasis cascade. Science321, 1785-1787, doi: 10.1126/science.1164853

Husemann, Y., Geigl, J. B., Schubert, F., Musiani, P., Meyer, M., Burghart, E., Forni, G., Eils, R., Fehm, T., Riethmuller, G., and Klein, C. A. (2008) Systemic spread is an early step in breast cancer. Cancer cell13, 58-68, doi: 10.1016/j.ccr.2007.12.003

Hahtola, S., Burghart, E., Puputti, M., Karenko, L., Abdel-Rahman, W. M., Vakeva, L., Jeskanen, L., Virolainen, S., Karvonen, J., Salmenkivi, K., Kinnula, V., Joensuu, H., Peltomaki, P., Klein, C. A., and Ranki, A. (2008) Cutaneous T-cell lymphoma-associated lung cancers show chromosomal aberrations differing from primary lung cancer. Genes Chromosomes Cancer47, 107-117, doi: 10.1002/gcc.20513

Hahtola, S., Burghart, E., Jeskanen, L., Karenko, L., Abdel-Rahman, W. M., Polzer, B., Kajanti, M., Peltomaki, P., Pettersson, T., Klein, C. A., and Ranki, A. (2008) Clinicopathological characterization and genomic aberrations in subcutaneous panniculitis-like T-cell lymphoma. The Journal of investigative dermatology128, 2304-2309, doi: 10.1038/jid.2008.6

Fuhrmann, C., Schmidt-Kittler, O., Stoecklein, N. H., Petat-Dutter, K., Vay, C., Bockler, K., Reinhardt, R., Ragg, T., and Klein, C. A. (2008) High-resolution array comparative genomic hybridization of single micrometastatic tumor cells. Nucleic Acids Res36, e39, doi: 10.1093/nar/gkn101

Weckermann, D., Polzer, B., and Klein, C. (2007) [Significance of cytokeratin positive cells in the bone marrow of patients with clinical localized prostate cancer]. Der Urologe. Ausg. A46, 1078-1080, doi: 10.1007/s00120-007-1432-4

Yekebas, E. F., Bogoevski, D., Bubenheim, M., Link, B. C., Kaifi, J. T., Wachowiak, R., Mann, O., Kutup, A., Cataldegirmen, G., Wolfram, L., Erbersdobler, A., Klein, C., Pantel, K., and Izbicki, J. R. (2006) Strong prognostic value of nodal and bone marrow micro-involvement in patients with pancreatic ductal carcinoma receiving no adjuvant chemotherapy. World J Gastroenterol12, 6515-6521, doi: 10.3748/wjg.v12.i40.6515

Klein, C. A., and Holzel, D. (2006) Systemic cancer progression and tumor dormancy: mathematical models meet single cell genomics. Cell cycle5, 1788-1798, doi: 10.4161/cc.5.16.3097

Klein, C. A. (2006) Random mutations, selected mutations: a PIN opens the door to new genetic landscapes. Proceedings of the National Academy of Sciences of the United States of America103, 18033-18034, doi: 10.1073/pnas.0609000103 

Hartmann, C. H., and Klein, C. A. (2006) Gene expression profiling of single cells on large-scale oligonucleotide arrays. Nucleic Acids Res34, e143, doi: 10.1093/nar/gkl740

Bahar, R., Hartmann, C. H., Rodriguez, K. A., Denny, A. D., Busuttil, R. A., Dolle, M. E., Calder, R. B., Chisholm, G. B., Pollock, B. H., Klein, C. A., and Vijg, J. (2006) Increased cell-to-cell variation in gene expression in ageing mouse heart. Nature441, 1011-1014, doi: 10.1038/nature04844

Wang, X. T., Sienel, W., Eggeling, S., Ludwig, C., Stoelben, E., Mueller, J., Klein, C. A., and Passlick, B. (2005) Detection of disseminated tumor cells in mediastinoscopic lymph node biopsies and lymphadenectomy specimens of patients with NSCLC by quantitative RT-PCR. Eur J Cardiothorac Surg28, 26-32, doi: 10.1016/j.ejcts.2005.03.029

Ulmer, A., Fischer, J. R., Schanz, S., Sotlar, K., Breuninger, H., Dietz, K., Fierlbeck, G., and Klein, C. A. (2005) Detection of melanoma cells displaying multiple genomic changes in histopathologically negative sentinel lymph nodes. Clin Cancer Res11, 5425-5432, doi: 10.1158/1078-0432.CCR-04-1995

Schardt, J. A., Meyer, M., Hartmann, C. H., Schubert, F., Schmidt-Kittler, O., Fuhrmann, C., Polzer, B., Petronio, M., Eils, R., and Klein, C. A. (2005) Genomic analysis of single cytokeratin-positive cells from bone marrow reveals early mutational events in breast cancer. Cancer cell8, 227-239, doi: 10.1016/j.ccr.2005.08.003

Klein, C. A., Zohlnhofer, D., Petat-Dutter, K., and Wendler, N. (2005) Gene expression analysis of a single or few cells. Curr Protoc Hum GenetChapter 11, Unit 11 18, doi: 10.1002/0471142905.hg1108s44

Klein, C. A. (2005) Single cell amplification methods for the study of cancer and cellular ageing. Mech Ageing Dev126, 147-151, doi: 10.1016/j.mad.2004.09.014

Ulmer, A., Schmidt-Kittler, O., Fischer, J., Ellwanger, U., Rassner, G., Riethmuller, G., Fierlbeck, G., and Klein, C. A. (2004) Immunomagnetic enrichment, genomic characterization, and prognostic impact of circulating melanoma cells. Clin Cancer Res10, 531-537, doi: 10.1158/1078-0432.CCR-0424-03

Stoecklein, N. H., Luebke, A. M., Erbersdobler, A., Knoefel, W. T., Schraut, W., Verde, P. E., Stern, F., Scheunemann, P., Peiper, M., Eisenberger, C. F., Izbicki, J. R., Klein, C. A., and Hosch, S. B. (2004) Copy number of chromosome 17 but not HER2 amplification predicts clinical outcome of patients with pancreatic ductal adenocarcinoma. Journal of clinical oncology : official journal of the American Society of Clinical Oncology22, 4737-4745, doi: 10.1200/JCO.2004.05.142

Klein, C. A. (2004) Gene expression sigantures, cancer cell evolution and metastatic progression. Cell cycle3, 29-31, doi: 10.4161/cc.3.1.634

Sienel, W., Hellers, J., Morresi-Hauf, A., Lichtinghagen, R., Mutschler, W., Jochum, M., Klein, C., Passlick, B., and Pantel, K. (2003) Prognostic impact of matrix metalloproteinase-9 in operable non-small cell lung cancer. International journal of cancer103, 647-651, doi: 10.1002/ijc.10841

Schmidt-Kittler, O., Ragg, T., Daskalakis, A., Granzow, M., Ahr, A., Blankenstein, T. J., Kaufmann, M., Diebold, J., Arnholdt, H., Muller, P., Bischoff, J., Harich, D., Schlimok, G., Riethmuller, G., Eils, R., and Klein, C. A. (2003) From latent disseminated cells to overt metastasis: genetic analysis of systemic breast cancer progression. Proceedings of the National Academy of Sciences of the United States of America100, 7737-7742, doi: 10.1073/pnas.1331931100

Klein, C. A. (2003) [From single disseminated tumor cells to metastasis insights from molecular genetic analyses of single cells]. Verh Dtsch Ges Pathol87, 158-164, PMID: 16888908

Klein, C. A. (2003) The systemic progression of human cancer: a focus on the individual disseminated cancer cell--the unit of selection. Adv Cancer Res89, 35-67, doi: 10.1016/s0065-230x(03)01002-9

Jentsch, I., Geigl, J., Klein, C. A., and Speicher, M. R. (2003) Seven-fluorochrome mouse M-FISH for high-resolution analysis of interchromosomal rearrangements. Cytogenet Genome Res103, 84-88, doi: 10.1159/000076294

Stoecklein, N. H., Erbersdobler, A., Schmidt-Kittler, O., Diebold, J., Schardt, J. A., Izbicki, J. R., and Klein, C. A. (2002) SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples. Am J Pathol161, 43-51, doi: 10.1016/S0002-9440(10)64155-7

Klein, C. A., Wilke, M., Pool, J., Vermeulen, C., Blokland, E., Burghart, E., Krostina, S., Wendler, N., Passlick, B., Riethmueller, G., and Goulmy, E. (2002) The hematopoietic system-specific minor histocompatibility antigen HA-1 shows aberrant expression in epithelial cancer cells. J Exp Med196, 359-368, doi: 10.1084/jem.20011838

Klein, C. A., Seidl, S., Petat-Dutter, K., Offner, S., Geigl, J. B., Schmidt-Kittler, O., Wendler, N., Passlick, B., Huber, R. M., Schlimok, G., Baeuerle, P. A., and Riethmuller, G. (2002) Combined transcriptome and genome analysis of single micrometastatic cells. Nat Biotechnol20, 387-392, doi: 10.1038/nbt0402-387

Klein, C. A., Blankenstein, T. J., Schmidt-Kittler, O., Petronio, M., Polzer, B., Stoecklein, N. H., and Riethmuller, G. (2002) Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet360, 683-689, doi: 10.1016/S0140-6736(02)09838-0

Klein, C. A. (2002) [Direct molecular analysis of single disseminated cancer cells: a prerequisite for the development of adjuvant therapies?]. Acta Med Austriaca Suppl59, 10-13, PMID: 12506754

Zohlnhofer, D., Richter, T., Neumann, F., Nuhrenberg, T., Wessely, R., Brandl, R., Murr, A., Klein, C. A., and Baeuerle, P. A. (2001) Transcriptome analysis reveals a role of interferon-gamma in human neointima formation. Mol Cell7, 1059-1069, doi: 10.1016/S1097-2765(01)00239-8

Zohlnhofer, D., Klein, C. A., Richter, T., Brandl, R., Murr, A., Nuhrenberg, T., Schomig, A., Baeuerle, P. A., and Neumann, F. J. (2001) Gene expression profiling of human stent-induced neointima by cDNA array analysis of microscopic specimens retrieved by helix cutter atherectomy: Detection of FK506-binding protein 12 upregulation. Circulation103, 1396-1402, doi: 10.1161/01.CIR.103.10.1396

Riethmuller, G., and Klein, C. A. (2001) Early cancer cell dissemination and late metastatic relapse: clinical reflections and biological approaches to the dormancy problem in patients. Semin Cancer Biol11, 307-311, doi: 10.1006/scbi.2001.0386

Klein, C. A. (2000) The biology and analysis of single disseminated tumour cells. Trends Cell Biol10, 489-493, doi: 10.1016/S0962-8924(00)01846-8

Riethmuller, G., Klein, C. A., and Pantel, K. (1999) Hunting down the seminal cells of clinical metastases. Immunol Today20, 294-296, doi: 10.1016/S0167-5699(98)01434-0

Klein, C. A., Schmidt-Kittler, O., Schardt, J. A., Pantel, K., Speicher, M. R., and Riethmuller, G. (1999) Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells. Proceedings of the National Academy of Sciences of the United States of America96, 4494-4499, doi: 10.1073/pnas.96.8.4494